The main vector of dengue fever and dengue haemorrhagic fever is Aedes aegypti in Myanmar. The use of chemicals is an effective way to reduce mosquito-borne viral disease transmission. Application of larvicides is the first stage of chemical control to kill the mosquitoes at the breeding site before disease transmission in community. This study was performed to assess the susceptibility status of Ae. aegypti larvae to temephos (0.02 mg/l diagnostic dosage and 1 mg/l applied dosage). Larval bioassays were conducted by using the WHO standard susceptibility procedures. Mosquito larvae were collected from Minteekin Ward in Aungmyaethasan Township, Mahamyain Ward in Mahaaungmyae Township and Keiksanamahe Ward in Chanayethasan Township from June to August, 2013.The 50% lethal times (LT50) for all strains of Ae. aegypti tested against diagnostic and applied dosages of temephos were found to be in the ranges between 77.00 to 109.74 minutes and 38.29 to 47.42 minutes, respectively. The mortality rates of Ae. aegypti larvae in all study areas were 100% to temephos, after 24 hours exposure. Based on the results, Ae. aegypti larvae in all areas are susceptible to temephos (diagnostic and applied dosages).